Introduction to Enzymes
Enzymes are biological catalysts. They speed up
chemical reactions, often very dramatically. For
example, carbonic anhydrase hydrates 1,000,000 CO2
molecules every second. Many biochemical reactions
would not occur at physiological temperatures and
pressures without enzymes. In short, enzymes allow
the processes of life to occur.
Virtually all known enzymes are proteins. Some RNA
molecules and carbohydrates have been shown to posses
catalytic activity, but the significance of this
in vivo is questionable.
Enzymes are characterised by their specificity for
their substrate; enzymes often recognise substrates
with superb accuracy. They also operate within defined
temperature and pH ranges and are often easily destroyed
by chemical reactions and heat.
Enzymes can be exploited by biotechnologists to
make many products for the food and pharmaceutical
industries.
Microbial enzymes are manufactured by growing the
microbial cells under specialized conditions so
that these cells produce their maximal amount of
active enzymes. It is important to control environmental
conditions during productions so that a high percentage
of these active catalysts are preserved intact.
Source of Enzyme:
Plant
Malted grains or tubers Amylase
Pineapple Bromelin (Protease)
Papaya Papain (Protease)
Microbial
Fungi (Molds and Yeast) amylase, protease, cellulase,
pectinase, lipase.
Bacteria
Amylase, protease, catalase, alkaline phosphatase
Application of these enzyemes:
1. Amylases, which catalyze the digestion of starch
into small segments of multiple sugars and into
individual soluble sugars.
2. Proteases, (or proteinase), which split up proteins
into their component amino acid building blocks.
3. Lipase, which split up animal and vegetable fats
and oils into their component part: glycerol and
fatty acids.
4. Cellulase (of various types) which breaks down
the complex molecule of cellulose into more digestible
components of single and multiple sugars.
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Manufacturing
of Enzymes, Herbal Extracts and Teaching Kits
List of products:
Enzymes (Microbial,Plant and Animal sources):
Alfa-amylase
Protease
Peroxidase
Catalase
Bromelian
Papain
Lipase
Acid phosphatase
Alkaline phosphatase
Cellulase
Urease
Trypsin
Lysozyme
Other Products:
Egg Albumin
BSA (Bovine Serum Albumin)
Herbal extracts::
Curcumin
Lycopene
Beta- carotene
Piperine
Nutmeg
Other compounds (organic acid):
Citric acid
Glutamic acid
Aflatoxin
SCP(Spirulina)
Immunology products:
Goat IgG
Mouse IgG
Bovine IgG
Human IgG
Sheep IgG
Chick IgG
Secondary conjugates.